Trace determination of gatifloxacin in pharmaceutical and biological samples using hydrophobic magnetic nanoparticles-assisted ionic liquid microextraction coupled with spectrofluorimetry

Motevalli, K.; Yaghoubi, Z.

doi:10.7508/ijnd.2013.01.003

Trace determination of gatifloxacin in pharmaceutical and biological samples using hydrophobic magnetic nanoparticles-assisted ionic liquid microextraction coupled with spectrofluorimetry

Document Type : Reasearch Paper

Authors

K. Motevalli ¹
Z. Yaghoubi ²

¹ Department of Applied Chemistry, fundamental sciences faculty , Islamic Azad University, South Tehran Branch, Tehran, Iran.

² Industrial faculty , Islamic Azad University, South Tehran Branch, Tehran, Iran.

10.7508/ijnd.2013.01.003

Abstract

A simple and rapid microextraction procedure based on the application of magnetic nanoparticles and hydrophobic 1-Hexylpyridinium hexafluorophosphate [Hpy][PF6] ionic liquid (IL) was applied to trace determination of gatifloxacin. A mixture of [Hpy][PF₆] and magnetic nanoparticles was injected into the sample solution containing the analyse of interest and a cloudy solution was formed. After centrifuging, the sediment phase containing enriched analyse was determined by spectrofluorimetry. Magnetic nanoparticles used in this microextraction procedure significantly improved the extraction recovery which is due to its proper hydrophobicity. [Hpy][PF₆] was used as the extraction solvent due to some physicochemical properties such as high hydrophobicity, water immiscibility and quite viscosity. Under optimum experimental conditions, the proposed method provided a limit of detection (LOD) of 0.09 µg L^-1 and a relative standard deviation (R.S.D.) of 3.0%. The present technique was applied to gatifloxacin determination in pharmaceutical formulations and human urine.

Keywords