The use of Gold nanorods conjugated with Herceptin in breast cancer treatment by photothermal therapy method in mouse model

Document Type : Reasearch Paper

Authors

• Mohsen Kalantari ¹
• Mojtaba Salouti ²
• Kazem Parivar ¹
• Mehrdad Hamidi ³
• Saeed Emadi ⁴

¹ Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

² Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran.

³ Zanjan Pharmaceutical Nanotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran.

⁴ Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, Iran.

Abstract

Treatment methods for breast cancer are not specific and each one has its own drawbacks. For this reason, scientists are seeking ways in which specifically affect cancer cells. Photothermal therapy is a method that uses near-infrared (NIR) laser energy to create sufficient heat to destroy cancer cells. In this study, the photothermal effect of gold nanorods (GNRs) was investigated for breast cancer treatment in vitro and in vivo. GNRs with the peak absorption of 808 nm were synthesized and conjugated with Herceptin (anti-HER2). After confirming the characteristics of the prepared conjugate, the therapeutic effect of the new agent was studied on SK-BR-3 cell line and BALB/c mouse model bearing breast tumor using the NIR laser. The cytotoxicity assay showed the biocompatibility of PEGylated GNRs-HER conjugate. Through the in vitro photothermal study, significant cell death was observed in breast cancer cells incubated with the conjugate along with the laser irradiation. Afterward, the biodistribution of the conjugate in the mouse model with breast tumor showed the considerable localization of new agent in breast tumor in comparison with other organs 24 h postinjection. The animal study showed a significant decrease in tumor growth rate as well as the increased lifespan of treated mice with breast tumor in comparison with the control groups.

Keywords

• Breast cancer
• Gold nanorods
• HER2 receptor
• Herceptin
• Photothermal therapy

20.1001.1.20088868.2018.9.2.3.9

Main Subjects

• Nano Bio Technology
• Nanomedicine

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References

 

[1]                 DeSantis C. E., Fedewa S. A., Goding Sauer A., Kramer J. L., Smith R. A., Jemal A., (2016), Breast cancer statistics, 2015: Convergence of incidence rates between black and white women. CA: A Cancer J. Clinicians. 66: 31-42.

[2]                 Abdoon A. S., Al-Ashkar E. A., Kandil O. M., Shaban A. M., Khaled H. M., El Sayed M. A., Hussein H. A., (2016), Efficacy and toxicity of plasmonic photothermal therapy (PPTT) using gold nanorods (GNRs) against mammary tumors in dogs and cats. Nanomedicine: Nanotechnol., Biology and Medicine. 12: 2291-2297.

[3]                 Srinivas P., Mounika G., (2011), Nanomedicine: The role of newer drug delivery technologies in cancer. Int. J. Nano Dimens. 2: 1-15.

[4]                 Huang X., El-Sayed M. A., (2011), Plasmonic photo-thermal therapy (PPTT). Alexandria J. Medic. 47: 1-9.

[5]                 Ahmad R., Fu J., He N., Li S., (2016), Advanced gold nanomaterials for photothermal therapy of cancer. J. Nanosci. Nanotechnol. 16: 67-80.

[6]                 Marsh M., Schelew E., Wolf S., Skippon T., (2009), Gold nanoparticles for cancer treatment. Queen's Univ. Kingston. 29.

[7]                 Wang J., Sui M., Fan W., (2010), Nanoparticles for tumor targeted therapies and their pharmacokinetics. Current Drug Metabol. 11: 129-141.

[8]                 Praetorius N. P., Mandal T. K., (2007), Engineered nanoparticles in cancer therapy. Recent Patents on Drug Del. Formul. 1: 37-51.

[9]                 Hwang S., Nam J., Jung S., Song J., Doh H., Kim S., (2014), Gold nanoparticle-mediated photothermal therapy: Current status and future perspective. Nanomedic. 9: 2003-2022.

[10]             Heidari Z., Salouti M., Sariri R., (2015), Breast cancer photothermal therapy based on gold nanorods targeted by covalently-coupled bombesin peptide. Nanotechnol. 26: 195101-195108.

[11]              Dickerson E. B., Dreaden E. C., Huang X., El-Sayed I. H., Chu H., Pushpanketh S., El-Sayed M. A., (2008), Gold nanorod assisted near-infrared plasmonic photothermal therapy (PPTT) of squamous cell carcinoma in mice. Cancer let. 269:  57-66.

[12]              Huang X., El-Sayed M. A., (2010), Gold nanoparticles: Optical properties and implementations in cancer diagnosis and photothermal therapy. J. Adv. Res. 1: 13-28.

[13]              Abadeer N. S., Murphy C. J., (2016), Recent progress in cancer thermal therapy using gold nanoparticles. J. Phys. Chem. C. 120: 4691-4716.

[14]              Singh M., Harris-Birtill D. C., Markar S. R., Hanna G. B., Elson D. S., (2015), Application of gold nanoparticles for gastrointestinal cancer theranostics: A systematic review. Nanomedicine: Nanotechnol., Biology and Medic. 11: 2083-2098.

[15]              Tolaney S. M., Krop I. E., (2009), Mechanisms of trastuzumab resistance in breast cancer. Anti-Canc. Agents in Medic. Chem. (Formerly Current Medicinal Chemistry-Anti-Cancer Agents). 9: 348-355.

[16]              Daniele L., Sapino A., (2009), Anti-HER2 treatment and breast cancer: State of the art, recent patents, and new strategies. Recent Patents on Anti-Canc. Drug Discov. 4: 9-18.

[17]              Leveque D., Gigou L., Bergerat J. P., (2008), Clinical pharmacology of trastuzumab. Current Clinical Pharmacol. 3: 51-55.

[18]              Widakowich C., Dinh P., Azambuja E. D., Awada A., Piccart-Gebhart M., (2008), HER-2 positive breast cancer: What else beyond trastuzumab-based therapy?. Anti-Cancer Agents in Medic. Chem. (Formerly Current Medicinal Chemistry-Anti-Cancer Agents). 8: 488-496.

[19]              Green H. N., Martyshkin D. V., Rodenburg C. M., Rosenthal E. L., Mirov S. B., (2011), Gold nanorod bioconjugates for active tumor targeting and photothermal therapy. J. Nanotechnol. 2011: Article ID 631753, 7 pages.

[20]              El-Sayed I. H., Huang X., El-Sayed M. A., (2006), Selective laser photo-thermal therapy of epithelial carcinoma using anti-EGFR antibody conjugated gold nanoparticles. Cancer Lett.239: 129-135.

[21]              Huang X., El-Sayed I. H., Qian W., El-Sayed M. A., (2006), Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods. J. Am. Chem. Soc.  128: 2115-2120.

[22]              Eghtedari M., Liopo A. V., Copland J. A., Oraevsky A. A., Motamedi M., (2008), Engineering of hetero-functional gold nanorods for the in vivo molecular targeting of breast cancer cells. Nano Lett. 9: 287-291.

[23]              Heidari Z., Sariri R., Salouti M., (2014), Gold nanorods-bombesin conjugate as a potential targeted imaging agent for detection of breast cancer. J. Photochem. Photobio. B: Biology. 130: 40-46.

[24]              Almaki J. H., Nasiri R., Idris A., Majid F. A. A., Salouti M., Wong T. S., Amini N., (2016), Synthesis, characterization and in vitro evaluation of exquisite targeting SPIONs–PEG–HER in HER²⁺ human breast cancer cells. Nanotechnol. 27: 105601-105608.

[25]              Liopo A., Conjusteau A., Oraevsky A., (2012), PEG-coated gold nanorod monoclonal antibody conjugates in preclinical research with optoacoustic tomography, photothermal therapy and sensing. Proc. SPIE. 8223: 822344-822349.

[26]              Wang Y., Black K. C., Luehmann H., Li W., Zhang Y., Cai X., Li Z. Y., (2013), Comparison study of gold nanohexapods, nanorods, and nanocages for photothermal cancer treatment. ACS Nano. 7: 2068-2077.

[27]              Jafari A., Salouti M., Shayesteh S. F., Heidari Z., Rajabi A. B., Boustani K., Nahardani A., (2015), Synthesis and characterization of Bombesin-superparamagnetic iron oxide nanoparticles as a targeted contrast agent for imaging of breast cancer using MRI. Nanotechnol. 26: 075101-075107.

[28]              Lu J., Owen S. C., Shoichet M. S., (2011), Stability of self-assembled polymeric micelles in serum. Macromolec. 44: 6002-6008.

[29]              Zhou F., Xing D., Ou Z., Wu B., Resasco D. E., Chen W. R., (2009), Cancer photothermal therapy in the near-infrared region by using single-walled carbon nanotubes. J. Biomed. Optic. 14: 021009-021009.

[30]              Hoffmann J., Bohlmann R., Heinrich N., Hofmeister H., Kroll J., Künzer H., Gieschen H., (2004), Characterization of new estrogen receptor destabilizing compounds: Effects on estrogen-sensitive and tamoxifen-resistant breast cancer. J. National Cancer Ins. 96: 210-218.

[31]              Shen  S., Tang H., Zhang X., Ren J., Pang Z., Wang D., Yang W., (2013), Targeting mesoporous silica-encapsulated gold nanorods for chemo-photothermal therapy with near-infrared radiation. Biomater: 34: 3150-3158.

[32]              Zhu H., Chen Y., Yan F. J., Chen J., Tao X. F., Ling J., Mao Z. W., (2017), Polysarcosine brush stabilized gold nanorods for in vivo near-infrared photothermal tumor therapy. Acta biomaterialia. 50: 534-545.

[33]              Liu Z., Cai W., He L., Nakayama N., Chen K., Sun X., Dai H., (2007), In vivo biodistribution and highly efficient tumour targeting of carbon nanotubes in mice. Nature Nanotechnol. 2: 47-52.

[34]              Yang M., Liu Y., Hou W., Zhi X., Zhang C., Jiang X., Cui D., (2017), Mitomycin C-treated human-induced pluripotent stem cells as a safe delivery system of gold nanorods for targeted photothermal therapy of gastric cancer. Nanoscale. 9: 334-340.

[35]              Chu M., Shao Y., Peng J., Dai X., Li H., Wu Q., Shi D., (2013), Near-infrared laser light mediated cancer therapy by photothermal effect of Fe₃O₄ magnetic nanoparticles. Biomater. 34: 4078-4088.

[36]              Shen S., Wang S., Zheng R., Zhu X., Jiang X., Fu D., Yang W., (2015), Magnetic nanoparticle clusters for photothermal therapy with near-infrared irradiation. Biomater. 39: 67-74.