Microwave-assisted greener synthesis of Silver nanoparticles using Entada rheedii leaf extract and investigation of its anticancer and antimicrobial properties

Document Type : Reasearch Paper

Authors

1 Department of Chemistry, Sree Neelakanta Government Sanskrit College, Pattambi, Kerala, India.

2 Department of Chemistry, Government Victoria College, Palakkad, Palakkad, Kerala, India.

3 Department of Zoology, Sree Neelakanta Government Sanskrit College, Pattambi, Palakkad, Kerala, India.

4 Department of Zoology, Sree Narayana College, Nattika, Thrissur, Kerala, India.

Abstract

In the current investigation phytochemically mediated, easy, efficient, and eco-friendly green synthesis of silver nanoparticles (AgNPs) was carried out using Entada rheedii leaf extract as a reducing and capping agent in a microwave-assisted synthetic pathway. UV-Visible spectroscopy, IR spectroscopy, scanning electron microscope (SEM), and transmission electron microscopic (TEM) techniques were used to confirm the formation of silver nanoparticles. The functional groups present in the capping agent were identified by FTIR analysis.  SEM and TEM analysis studied the surface morphology of the biosynthesized AgNPs. AgNPs also showed significant antibacterial effects against four different bacterial pathogens Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Vibrio cholera. Additionally, the prepared AgNPs exhibited solid anticancer activity against Dalton's lymphoma ascites (DLA) cells.

Keywords


[1] Doane T. L., Burda C., (2012), The unique role of nanoparticles in nanomedicine: Imaging, drug delivery and therapy. Chem. Soc. Rev. 41: 2885–2911.
[2] Francis S., Joseph S., Koshy E. P., Mathew B., (2018), Microwave assisted green synthesis of silver nanoparticles using leaf extract of elephantopus scaber and its environmental and biological applications. Artif. Cells Nanomed. Biotechnol. 46: 795–804.
[3] Rajan A., Vilas V., Philip D., (2015), Studies on catalytic, antioxidant, antibacterial and anticancer activities of biogenic gold nanoparticles. J. Mol. Liq. 212: 331–339.
[4] Roopan S. M., Surendra T. V., Elango G., Kumar S. H. S., (2014), Biosynthetic trends and future aspects of bimetallic nanoparticles and its medicinal applications. Appl. Microbiol. Biotechnol. 98: 5289–5300.
[5] Tian K., Prestgard M., Tiwari A., (2014), A review of recent advances in nonenzymatic glucose sensors. Mater. Sci. Eng. C. 41: 100–118.
[6] Yan Y., Wang T., Li X., Pang H., Xue H., (2017), Noble metal-based materials in high-performance supercapacitors. Inorg. Chem. Front. 4: 33–51.
[7] Joseph S., Mathew B., (2015), Microwave assisted facile green synthesis of silver and gold nanocatalysts using the leaf extract of Aerva lanata. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 136: 1371–1379.
[8] Nakkala J. R., Mata R., Gupta A. K., Sadras S. R., (2014), Biological activities of green silver nanoparticles synthesized with Acorous calamus rhizome extract. Eur. J. Med. Chem. 85: 784–794.
[9] Chan Y. S., Mat Don M., (2013), Biosynthesis and structural characterization of Ag nanoparticles from white rot fungi. Mater. Sci. Eng. C. 33: 282–288.
[10] Jadoun S., Arif R., Jangid N. K., Meena R. K., (2021), Green synthesis of nanoparticles using plant extracts: A review. Environ. Chem. Lett. 19: 355–374.
[11] Joseph S., Mathew B., (2015), Facile synthesis of Silver nanoparticles and their application in dye degradation. Mater. Sci. Eng. B. 195: 90–97.
[12] K. Kumar S., Amuta R., Arumugam P., Berchmans S., (2011), Synthesis of Gold nanoparticles: An ecofriendly approach using hansenula anomala. ACS Appl. Mater. Interf.  3: 1418–1425.
[13] Narayanan K. B., Sakthivel N., (2010), Biological synthesis of metal nanoparticles by microbes. Adv. Colloid Interf. Sci. 156: 1–13.
[14] Sadeghi B., Jamali M., Kia Sh., Amini Nia A., Ghafari S., (2010), Synthesis and characterization of Silver nanoparticles for antibacterial activity.  Int. J. Nano Dimens. 1: 119–124.
[15] Sadeghi B., Mohammadzadeh M., Babakhani B., (2015), Green synthesis of gold nanoparticles using Stevia rebaudiana leaf extracts: Characterization and their stability. J. Photochem. Photobiol. B. 148: 101–106.
[16] Sadeghi B., Gholamhoseinpoor F., (2015), A study on the stability and green synthesis of silver nanoparticles using Ziziphora tenuior (Zt) extract at room temperature. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 134: 310–315.
[17] Singh P., Mijakovic I., (2022), Strong antimicrobial activity of Silver nanoparticles obtained by the green synthesis in Viridibacillus sp. Extracts. Front. Microbiol. 13: 820048.
[18] Kudle K., Donda M., Merugu R., Kudle M., Pratap M., (2013), Microwave assisted green synthesis of silver nanoparticles using Boswellia Serrata flower extract and evaluation of their antimicrobial activity. Int. Res. J. Pharm. 4: 197–200.
[19] Perveen K., Husain F. M., Qais F. A., Khan A., Razak S., Afsar T., Alam P., Almajwal A. M., Abulmeaty M. M. A., (2021), Microwave-assisted rapid green synthesis of Gold nanoparticles using seed extract of trachyspermum ammi: ROS mediated biofilm inhibition and anticancer activity. Biomolecules. 11: 197-202.
[20] Saidu F. K., Mathew A., Parveen A., Valiyathra V., Thomas G. V., (2019), Novel green synthesis of silver nanoparticles using clammy cherry (Cordia obliqua Willd) fruit extract and investigation on its catalytic and antimicrobial properties. SN Appl. Sci. 1: 1368-1372.
[21] Nathan V., (2017), Phytochemical evaluation and antimicrobial efficacy of Entada rheedii Spreng. Glob. J. Res. Anal. 6: 624–625.
[22] Jain S., Mehata M. S., (2017), Medicinal plant leaf extract and pure flavonoid mediated green synthesis of silver nanoparticles and their enhanced antibacterial property. Sci. Rep. 7: 15867-15872.
[23] Sadeghi B., Rostami A., Momeni S. S., (2015), Facile green synthesis of silver nanoparticles using seed aqueous extract of Pistacia atlantica and its antibacterial activity. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 134: 326–332.
[24] Krishnaraj C., Jagan E. G., Rajasekar S., Selvakumar P., Kalaichelvan P. T., Mohan N., (2010), Synthesis of Silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water borne pathogens. Colloids Surf. B. Biointerf. 76: 50–56.