Cytotoxic properties (MDA-MB-231- an epithelial breast cancer cell line) and bactericidal activity of Silver nanoparticles mediated by Strobilanthes ciliata nees

Document Type : Reasearch Paper

Authors

• Vasanth - Nayagam ^{1, 2}
• Abitha Ponnambalam ^{1, 2}
• Joelri Michael - Raj Lourdhurajan ¹
• Anthony Samy ¹
• Savarimuthu Ignacimuthu ³

¹ Dept of Botany, St. Xavier’s College, Palayamkottai, Tirnelveli, Tamilnadu, India (Affiliated to Manonmaniam Sundaranar University).

² Dept of Botany, Alagappa Univeristy,Karaikudi, Tamilnadu, India.

³ The Anna and Donald Waite Chair, Creighton University, Omaha, USA.

10.22034/ijnd.2023.1995048.2252

Abstract

The sciences that intrigue about nanotechnology are increased ever before and improved permeability in its applications at various arenas. Precisely, the Silver nanoparticles (AgNPs) implication is deep-time perspective to understand that fascinating nanomaterials are involved in different biomedical applications. Since NPs application derivates are physical and chemical based but biological methods is still holds a profound impact. Hence, the medicinal plant Strobilanthes ciliata was utilized to synthesis AgNPs and the same was subjected to UV irradiation. And subsequent application of the crude biosynthesis of AgNPs was experimented with various physico-chemical parameters. Biosynthesized particle characterization was carried out by color pattern and UV-visible spectroscopy and followed by FTIR, SEM and finally experimenting with cancer cells as cyto-toxic study. The characterization result of particle size of synthesized AgNPs is 77 nm. The highest peak obtained in FTIR is at 3439.48cm−1 (OH) and the UV range is 477 nm. As far as the cancer cells are concerned, the cell survival rate at 1 mcg is 62% and at 1.5 mcg is 30% which is optimal. The lattice planes of XRD are face-centered-cubic (FCC) structure. The Particle size of S.ciliata mediated AgNPs is 77.7 nm. Biological methods attain much concern and forethought because bio-resources are used as precursors in synthesis of NPs. Therefore, herein we exclusively have done the plant-mediated metallic NPs synthesis, characterization and applications in detailed summary.  The present research observation will hold enrich understanding about AgNPs interaction and the mode of synthesis with various expertise and subject.

Keywords

• Cancer Cells
• Characterization
• Green Chemistry
• Nanomaterials
• Strobilanthes Ciliata

20.1001.1.20088868.2023.14.4.7.2

Main Subjects

• Nano Bio Technology

---

References

[1] Bray F., Ferlay J., Soerjomataram I., Siegel R. L., Torre L. A., Jemal A., (2018), Global cancer statistics GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J. Clin. 68: 394-424. https://doi.org/10.3322/caac.21492

[2] Han H., (2017), Hypertension and breast cancer risk: A systematic review and meta-analysis. Sci. Rep. 7: 1-9. https://doi.org/10.1186/s12645-022-00137-8

[3] Jemal A., Bray F., Ferly J., Mellisa M., Ward E. M., (2011), Global cancer facts & figures. American Cancer Society. A Cancer J. Clinic. 61: 69-90. https://doi.org/10.3322/caac.20107

[4] Iqbal M. J., Rashid U., Javed Z., (2022), Biosynthesized silver nanoparticles and miR34a mimics mediated activation of death receptor in MCF-7 human breast cancer cell lines. Cancer Nano. 13: 31-36. https://doi.org/10.1186/s12645-022-00137-8

[5] Gaur U., Aggarwal B. B., (2003), Regulation of proliferation, survival and apoptosis by members of the TNF superfamily. Biochem Pharmacol. 66: 1403-1408. https://doi.org/10.1186/s12645-022-00137-8

[6] Tabrez S., Priyadarshini M., Urooj M., Shakil S., Md Ashraf G., Shahnawaz K. M., Kamal M. A., Alam Q., Jabir N. R., Abuzenadah A. M., Chaudhary A. G. A., Damanhouri G. A., (2013), Cancer chemoprevention by polyphenols and their potential application as nanomedicine. J. Environ. Sci. Health Part C. Environ. Carcinog. Ecotoxicol. Rev. 31: 67-98. https://doi.org/10.1080/10590501.2013.763577

[7] Ahmed N., Gupta A., Kumar., Nimesh S., (2016), Antibacterial efficacy of silver nanoparticles synthesized employing Terminalia arjuna bark extract Artif. Cells Nanomed. Biotechnol. 45: 1-9. https://doi.org/10.1080/21691401.2016.1215328

[8] Roy C., Sarkar A., Ghosh C., (2015), Photocatalytic activity of biogenic silver nanoparticles synthesized using potato (Solanum tuberosum) infusion. Biomol. Spectrosc. 146: 286-291. https://doi.org/10.1016/j.saa.2015.02.058

[9] Saravanakumar., (2016), Low-cost and eco-friendly green synthesis of silver nanoparticles using Prunus japonica (Rosaceae) leaf extract and their antibacterial, antioxidant properties. Artif. Cells Nanomed. Biotechnol. 45: 1-7. https://doi.org/10.1080/21691401.2016.1203795

[10] Eun Young A., Sang Woo Sh., Kyeongsoon K., Youmie Park F., (2022), Green synthesis of Titanium dioxide nanoparticles by upcycling mangosteen (Garcinia mangostana) pericarp extract. Nanoscale Res. Lett. 17: 1-12. https://doi.org/10.1186/s11671-022-03678-4

[11] Dasari S., (2013), Biosynthesis, characterization, antibacterial and antioxidant activity of silver nanoparticles produced by lichens. J. Bionanosci.7: 237-242. https://doi.org/10.1166/jbns.2013.1140

[12] Chauhan N., Tyagi A. K., Kumar P., Malik A., (2016), Antibacterial potential of Jatropha curcas synthesized silver nanoparticles against food borne pathogens. Front Microbiol. 7: 1-13. https://doi.org/10.3389/fmicb.2016.01748

[13] Krupa A. N. D., Raghavan V., (2014), Biosynthesis of silver nanoparticles using Aegle marmelos (bael) fruit extract and its application to prevent adhesion of bacteria: A strategy to control microfouling. Bioinorg. Chem. Appl. Article ID 949538. https://doi.org/10.1155/2014/949538

[14] Vasanth N., Melchias G., Kumaravel P., (2018), Green synthesis of silver nanoparticles mediated by coccinia grandis and Phyllanthus emblica: A comparative comprehension. Appl. Nanosc. 8: 205-219. https://doi.org/10.1007/s13204-018-0739-3

[15] Ankanna S., Prasad T. N., Elumalai E. K., Savithramma N., (2010), Production of biogenic silver nanoparticles using Boswellia ovalifoliolata stem bark. Digest. J. Nanomater. Biostruct. 5: 369-372.

[16] Pavani K., Divya V., Veena V., Adity I., Devakinandan M., (2014), Influence of bioengineered zinc nanoparticles and Zinc metal on Cicer arietinum seedlings growth. Asian J. Agri. Biol. 2: 216-223.

[17] Hazhir N., Chekin F., Bakhsh Raoof J., Fathi Sh., (2020), Anticancer activity of doxorubicin conjugated to polymer/carbon basednanohybrid against MCF-7 breast and HT-29 colon cancer cells . Int. J. Nano Dimens. 12: 11-19.

[18] Tayarani N., Mousavi S. H., Asghari M., Sadeghnia H. R., (2010), Protective effect of Nigella sativa extract and thymoquinone on serum/glucose deprivation-induced PC12 cells death. Cell. Molec. Neurobiol. 30: 591-598. https://doi.org/10.1007/s10571-009-9484-1

[19] Daysi Diaz D., Madadi Gholipour H., Bazian M., Thapa B., Beltran Huarac J., (2022), Photodynamic therapeutic effect of nanostructured metal sulfde photosensitizers on cancer treatment. Nanoscale Res. Lett. 17: 1-24. https://doi.org/10.1186/s11671-022-03674-8

[20] Verma P., Maheshwari S. K., (2019), Applications of silver nanoparticles in diverse sectors. Int. J. Nano Dimens. 10: 18-36.

[21] Ohnishi Takako K., Masanori I., Kazuya I., Sunhwa K., Ayako S., Eiji K., Toshio I., Takashi M., Tatsuhiko K., Tadatsugu T., Hiroshi T., Toshiyuki T., (2004), Costimulatory signals mediated by the ITAM motif cooperate with RANKL for bone homeostasis. Nature. 428: 758-763. https://doi.org/10.1038/nature02444